Nikon Microinjection Scope

The MIH core is equipped with a Nikon Eclipse E600FN IR electrophysiology / microinjection scope with fluorescent capabilities, for use in microinjection and slice imaging experiments. Its wide aperture water immersion lenses are also suitable for a variety of live explant imaging studies. The scope is equipped with DAPI, TRITC, Texas Red and Cy5/DiD filter cubes, as well as DIC capabilities. The system is equipped with a standard air table, and additional XY anti-vibration platform to further dampen microscope versus platform vibrations. The stainless steel Gibraltar stage is drilled for 1/4" mounts. Workstation includes micromanipulators and nano- and piezo- injector modules for use in cell and pronuclear injection studies. Standard electrophysiologic simulating and recording units are also integrated into the workstation.

Images can be acquired on this microscope through either CCD, 35mm digital, or 35mm film cameras. The workstation is equipped with a variety of morphometry / image analysis software.   In addition to departmental and SSH secure shell network connections from the workstation, a 52x CD burner and high speed USB ports are provided for users to download their data.   Data from this system can also be directly ported to the remote imaging workstation to allow users to perform more detailed image analyses. Security is provided by video surveillance in operation 24 hours / day. Please insure that your training is complete and you have a recent copy of the user notes  prior to using this scope.

To setup an account for this system, fill out the following form, and see deposit conditions which apply. The completed form may be emailed to the facility administrator. You will be sent your MIH ID and password via email once the account is established.

Internal Academic (Pharmacy):                       $20/hr
External Academic (University of Toronto):     $40/hr
Non-Academic:                                                $60/hr

To book time, click on the link shown below.   Remember to enter your six digit ID code to validate bookings.   Individual lab groups should limit their time to a maximum of three hours per day; two hours during peak periods.  Times may be booked 7 days per week, 24 hours per day.

     Calender for microinjection       (Account: mih_microinjection)

     MIH Home Page

     Faculty of Pharmacy, University of Toronto 

     University of Toronto Home Page 

     Henderson Laboratory Home Page

      Send mail to Dr. Henderson:    jeff.henderson@utoronto.ca